Grade : CR1 (Chargé de Recherche de 1ère classe)
CRNL WAKING TEAM
Equipe Physiologie intégrée du système d'éveil,
Internet : http://www.invivobiosensor.fr/
Publications dans les bases de données en ligne :
Microelectrode biosensors are excellent tools for monitoring the concentration of neurotransmitters and metabolites in the brain with high temporal resolution and minimal tissue injury. The lab engineers platinum microelectrode-based biosensors using oxidase enzymes for molecular recognition. Typical biosensors detect glucose, lactate, glutamate, D-serine and peroxide in the brain. The lab seeks to develop smaller biosensors and to detect new molecules with neurobiological relevance.
Regulation of neurotransmitter extracellular concentration:
The lab focuses on the biochemical mechanisms that regulate the extracellular concentration of glutamate and D-serine in the brain. These transmitters can produce neuronal injury when present in high extracellular concentrations, through excitotoxic mechanisms. Determining the range of their physiological concentrations and the threshold above which they become toxic is an important challenge in both neuroscience and analytical chemistry.
Neurotoxic mechanisms in brain diseases:
The lab focuses on the detection of neurotoxic mechanisms that are initiated during pathological conditions such as brain trauma, epilepsy, subarachnoid hemorrhage or ischemia. Glutamate and/or D-serine detection can reveal excitotoxic processes, whereas peroxide is an end product of reactive oxygen species (ROS) released during oxidative stress. Microelectrode biosensors can provide key information about when, where and how much of these toxic molecules are released during neuronal injury.
Microelectrode Biosensors; Series: Neuromethods, Vol. 80
Marinesco, Stephane; Dale, Nicholas (Eds.)
Maucler C, Pernot P, Vasylieva N, Pollegioni L and Marinesco S (2013) In vivo D-serine hetero-exchange through alanine-serine-cysteine (ASC) transporters detected by microelectrode biosensors. ACS Chem Neurosci. 4: 772-81.
N. Vasylieva, C. Maucler, A. Meiller, H. Viscogliosi, T. Lieutaud, D. Barbier and S. Marinesco (2013) An immobilization method for biosensors with preserved enzyme specificity: consequences for glutamate detection in the central nervous system. Anal Chem 85: 2507-15.
P. Pernot, C. Maucler, Y. Tholance, N. Vasylieva, G. Debilly, L. Pollegioni, R. Cespuglio and S. Marinesco (2012) D-serine diffusion through the blood-brain barrier: effect on D-serine compartmentalization and storage. Neurochem Int. 60: 837-45.
N. Vasylieva, B. Barnych, A. Meiller, C. Maucler, L. Pollegioni, D. Barbier and S. Marinesco (2011) Covalent enzyme immobilization by poly(ethylene glycol) diglycidyl ether (PEGDE) for microelectrode biosensor preparation. Biosens Bioelectron, 26(10):3993-4000.
P. Pernot, J.-P. Mothet, O. Schuvailo, A. Soldatkin, L. Pollegioni, M.Pilone, M.-T. Adeline, R. Cespuglio and S. Marinesco (2008) Characterization of a yeast D-amino acid oxidase microbiosensor for D-serine detection in the central nervous system. Anal Chem. 80(5):1589-1597.
S. Marinesco, N. Wickremasinghe and T.J. Carew (2006) Regulation of behavioral and synaptic plasticity by serotonin release within local modulatory fields in the central nervous system of Aplysia. J Neurosci, 26: 12682-12693.
L.G. Gruenbaum, D.M. Gilligan, M.R. Picciotto, S. Marinesco and T.J. Carew (2003) Identification and functional characterization of ApADD, an Aplysia cytoskeletal protein homologous to mammalian adducins: Increased PKC phosphorylation during long-term synaptic facilitation. J Neurosci; 23: 2675-2685.
S.Marinesco and T.J.Carew (2002) Serotonin release evoked by tail-nerve shock in Aplysia: characterization and relationship to heterosynaptic plasticity. J Neurosci; 22: 2299-2312.
I graduated from Ecole Polytechnique in 1992, and received my PhD from Universite Claude Bernard Lyon I (Lyon, France) in Michel Jouvet's laboratory.vI was then a postdoctoral fellow in Dr. Thomas J. Carew's laboratory at Yale University (1999-2000) and at the University of California Irvine (2001-2004). My research focus was on learning and memory in the marine snail Aplysia californica. I studied the modulation of glutamatergic sensorimotor synapses by serotonin during learning and developed an electrochemical detection technique for monitoring serotonin release in real time in Aplysia ganglia. I joined the department of Cellular and Molecular Neurobiology in Gif sur Yvette (France) in 2004 to develop a D-serine microbiosensor and study neuron-glia interactions. I am now a researcher in the Lyon Neuroscience Center (CRNL, team Waking), and director of the AniRA-Neurochem technological platform at University Claude Bernard Lyon I.
|Dernière modification le Jeudi 17 Octobre 2013 à 12:15:26.|